Journal: Cell Death & Disease

Article Title: Downregulation of miR-205 and miR-31 confers resistance to chemotherapy-induced apoptosis in prostate cancer cells

doi: 10.1038/cddis.2010.85

Figure Lengend Snippet: miR-205 targets Bcl-w and miR-31 targets E2F6. ( a ) Upper panel, predicted duplex formation between human BCL2L2 3′UTR and miR-205. Lower panel, predicted duplex formation between human E2F6 3′UTR and miR-31. ( b ) Left, WPE1-NB26 cells were transfected with GFP- BCL2L2 ( 3′UTR) or GFP- BCL2L2 (3′UTR)-mut plasmids together with plasmids expressing miR-205 or a negative control miRNA. Western blotting was performed with anti-GFP and anti-actin antibodies. Right, WPE1-NB26 cells were transfected with GFP- E2F6 ( 3′UTR) or GFP- E2F6 (3′UTR)-mut plasmids together with plasmids expressing miR-31 or a negative control miRNA. Western blotting was performed with anti-GFP and anti-actin antibodies. ( c ) Proteins were isolated from the indicated cell lines, western blots were done using indicated antibodies. ( d ) Left, WPE1-NB26 cells were transfected with pcDNA6.2-GW/EmGFP-miR-negative control or pcDNA6.2-GW/EmGFP-miR205, or pcDNA6.2-GW/EmGFP-miR31 plasmids. Expression of miRNAs was confirmed by real-time PCR. At 48 h after transfection, cell lysates were analyzed by western blots with the indicated antibodies (Middle). Right, WPE1-NA22 cells were transfected with synthetic miRNA inhibitors specific to miR-205 or miR-31, or the negative control inhibitor. At 48 h after transfection, cell lysates were analyzed by western blots with the indicated antibodies. Representative images of three independent experiments were shown

Article Snippet: To determine whether differential miRNA expression has a role in the apoptosis resistance in WPE1-NB26 cells, we compared miRNA expression profiles between WPE1-NA22 and WPE1-NB26 cells, using the mir Vana miRNA Bioarray.

Techniques: Transfection, Expressing, Negative Control, Western Blot, Isolation, Real-time Polymerase Chain Reaction

Journal: Cell Death & Disease

Article Title: Downregulation of miR-205 and miR-31 confers resistance to chemotherapy-induced apoptosis in prostate cancer cells

doi: 10.1038/cddis.2010.85

Figure Lengend Snippet: miR-205 and miR-31 sensitize WPE1-NB26 cells to chemotherapy-induced apoptosis. ( a ) WPE1-NB26 cell line stably expressing miR-205 was established and miR-205 expression was confirmed by real-time PCR. Negative control miRNA or miR-205 expressing cells were treated with various doses of Docetaxel for 24 h, and apoptosis was measured by Cell Death Detection Elisa analysis as described in Materials and Methods section. ( b ) Negative control or miR-205 expressing WPE1-NB26 cells were treated with various doses of Cisplatin for 24 h, and apoptosis was detected as above. ( c ) WPE1-NB26 cell line stably expressing miR-31 was established and miR-31 expression was confirmed by real-time PCR. Negative control miRNA or miR-31 expressing cells were treated with various doses of Docetaxel for 24 h, and apoptosis was measured as above. ( d ) Negative control miRNA or miR-31 expressing WPE1-NB26 cells were treated with various doses of Cisplatin for 24 h, and apoptosis was detected as above. The experiments have been repeated three times, data shown are mean values+S.D. ( * P <0.05, *** P <0.001)

Article Snippet: To determine whether differential miRNA expression has a role in the apoptosis resistance in WPE1-NB26 cells, we compared miRNA expression profiles between WPE1-NA22 and WPE1-NB26 cells, using the mir Vana miRNA Bioarray.

Techniques: Stable Transfection, Expressing, Real-time Polymerase Chain Reaction, Negative Control, Enzyme-linked Immunosorbent Assay

Journal: Oncotarget

Article Title: Identification of microRNA differentially expressed in three subtypes of non-small cell lung cancer and in silico functional analysis

doi: 10.18632/oncotarget.20218

Figure Lengend Snippet: Heat map of the significantly dysregulated miRNAs between the cancer and normal tissue samples in GSE19945 (A) , GSE51852 (B) and GSE25508 (C) . (D) ( Upper ) Venn diagram between the up-regulated and down-regulated miRNAs from each dataset. ( Lower ) the lists of the common dysregulated miRNAs. Red: up-regulation; blue: down-regulation.

Article Snippet: GSE16025 dataset contained 61 SCC samples and 10 matched normal lung samples and profiled on MirVana miRNA Bioarrays (version 2, Ambio).

Techniques:

Journal: Oncotarget

Article Title: Identification of microRNA differentially expressed in three subtypes of non-small cell lung cancer and in silico functional analysis

doi: 10.18632/oncotarget.20218

Figure Lengend Snippet: (A) Overlapped DEmiRNAs in ADC were presented as Venn diagram. RRA analysis of up-regulated (B) and down-regulated miRNAs (C) to identify common DEmiRNAs in ADC. (D) Overlapped DEmiRNAs in SCC were presented. RRA analysis of up-regulated (E) and down-regulated miRNAs (F) in SCC were listed. (G) Overlapped DEmiRNAs in LCLC were presented. RRA analysis of up-regulated (H) and down-regulated miRNAs (I) in LCLC were listed. For the RRA column diagram, red: crude p-value (up-regulation); Dark red: corrected p-value (up-regulation). Blue: crude p-value (down-regulation); Dark blue: corrected p-value (down-regulation).

Article Snippet: GSE16025 dataset contained 61 SCC samples and 10 matched normal lung samples and profiled on MirVana miRNA Bioarrays (version 2, Ambio).

Techniques:

Journal: Oncotarget

Article Title: Identification of microRNA differentially expressed in three subtypes of non-small cell lung cancer and in silico functional analysis

doi: 10.18632/oncotarget.20218

Figure Lengend Snippet: Venn diagram of overlapped dysregulated miRNAs of SCC vs. LCLC (A) and SCC vs. ADC (C) . Common DEmiRNAs of SCC vs. LCLC (B) and SCC vs. ADC (D) were identified using RRA method. For the RRA column diagram, red: rude p-value; blue: corrected p-value.

Article Snippet: GSE16025 dataset contained 61 SCC samples and 10 matched normal lung samples and profiled on MirVana miRNA Bioarrays (version 2, Ambio).

Techniques:

Journal: Oncotarget

Article Title: Identification of microRNA differentially expressed in three subtypes of non-small cell lung cancer and in silico functional analysis

doi: 10.18632/oncotarget.20218

Figure Lengend Snippet: (A) The interaction between three miRNAs (miR-203, miR-205 and miR-375) and of their predicted target genes. The central yellow circles represented miRNAs. The color of the circles with the names of the target genes on it varies according to their cumulative weighted context++ score. KEGG pathway enrichments of predicted target genes of miR-203 (B) , miR-205 (C) and miR-375 (D) , were presented, respectively. (E) The combinatorial effects of three miRNAs in KEGG pathways were predicted.

Article Snippet: GSE16025 dataset contained 61 SCC samples and 10 matched normal lung samples and profiled on MirVana miRNA Bioarrays (version 2, Ambio).

Techniques:

Journal: Oncotarget

Article Title: Identification of microRNA differentially expressed in three subtypes of non-small cell lung cancer and in silico functional analysis

doi: 10.18632/oncotarget.20218

Figure Lengend Snippet: (A) The expression profile of 3 miRNAs (miR-203, miR-205 and miR-375) and 90 Hippo signaling related genes was presented as heatmap. (B) Pearson correlation for each miRNA and 90 Hippo signaling related genes. The genes which considered to have statistically significant relationship with the miRNA were marked red. (C) The interaction network of the significant couples derived from Pearson correction.

Article Snippet: GSE16025 dataset contained 61 SCC samples and 10 matched normal lung samples and profiled on MirVana miRNA Bioarrays (version 2, Ambio).

Techniques: Expressing, Derivative Assay